Journal: Molecular Neurodegeneration

Article Title: No dramatic age-related loss of hair cells and spiral ganglion neurons in Bcl-2 over-expression mice or Bax null mice

doi: 10.1186/1750-1326-5-28

Figure Lengend Snippet: Overexpression of human bcl-2 transgene in the mouse cochlea at four months old . (A) Immunoblot analysis of human Bcl-2 expression. (1) A sample from two cochleae of C57BL/6J; (2) a sample from the transgenic mice. (B) Immunostaining of calretinin (CR) human bcl-2 in the cochlea of a transgenic mouse.

Article Snippet: 10 μm cryostat sections were cut and immunostained with monoclonal hamster anti-human Bcl-2 antisera (1:100, PharMingen).

Techniques: Over Expression, Western Blot, Expressing, Transgenic Assay, Immunostaining

Journal: Molecular Neurodegeneration

Article Title: No dramatic age-related loss of hair cells and spiral ganglion neurons in Bcl-2 over-expression mice or Bax null mice

doi: 10.1186/1750-1326-5-28

Figure Lengend Snippet: Comparison of age-related hearing loss between wild-type and bcl-2 transgenic mice . Mean (+SD) ABR thresholds were measured in wild type and BCL-2 transgenic mice versus age (2 and 9 months) and stimulus frequency. Five female mice were used for each group. No significant threshold protection effect of the BCL-2 transgene was apparent.

Article Snippet: 10 μm cryostat sections were cut and immunostained with monoclonal hamster anti-human Bcl-2 antisera (1:100, PharMingen).

Techniques: Transgenic Assay

Journal: Molecular Neurodegeneration

Article Title: No dramatic age-related loss of hair cells and spiral ganglion neurons in Bcl-2 over-expression mice or Bax null mice

doi: 10.1186/1750-1326-5-28

Figure Lengend Snippet: Comparison of age-related loss of hair cells between wild-type and bcl-2 transgenic mice . Mean (+SD) inner and outer hair cell density at four basal-apical locations versus age (18 and 24 months) and BCL-2 genotype. Averaged data for 2 month-old control female mice (n = 5), pooled by genotype, provided normal comparison numbers. Both wild type (n = 4) and transgenic mice (n = 4) showed progressive loss of inner (IHCs) and outer hair cells (OHCs), beginning in the base. There was no significant tendency for BCL-2 transgenic to retain hair cells.

Article Snippet: 10 μm cryostat sections were cut and immunostained with monoclonal hamster anti-human Bcl-2 antisera (1:100, PharMingen).

Techniques: Transgenic Assay

Journal: Molecular Neurodegeneration

Article Title: No dramatic age-related loss of hair cells and spiral ganglion neurons in Bcl-2 over-expression mice or Bax null mice

doi: 10.1186/1750-1326-5-28

Figure Lengend Snippet: Comparison of age-related loss of hair cells and SGNs between wild-type and bcl-2 transgenic mice . Histological sections of spiral ganglia of wild-type (WT) and transgenic mice (Bcl-2) from 2 or 12 month-old age groups were shown. There were four animals in each group. The spiral ganglia at the base are shown in the left panel, and the ganglia in the apex at the right panel. SGNs were counted from each age group and presented below the histological sections.

Article Snippet: 10 μm cryostat sections were cut and immunostained with monoclonal hamster anti-human Bcl-2 antisera (1:100, PharMingen).

Techniques: Transgenic Assay

Journal: Molecular Neurodegeneration

Article Title: No dramatic age-related loss of hair cells and spiral ganglion neurons in Bcl-2 over-expression mice or Bax null mice

doi: 10.1186/1750-1326-5-28

Figure Lengend Snippet: Comparison of age-related hearing loss between wild-type and Bax-/- mice . Mean (+SD) ABR thresholds were measured in both wild type (n = 3) and Bax-/- mice (n = 4). They were 5 month-old male mice. No significant threshold protection effect of the BCL-2 transgene was apparent.

Article Snippet: 10 μm cryostat sections were cut and immunostained with monoclonal hamster anti-human Bcl-2 antisera (1:100, PharMingen).

Techniques:

Journal: bioRxiv

Article Title: Lesion-specific suppression of YAP/TAZ by biomimetic nanodrug ameliorates atherosclerosis development

doi: 10.1101/2023.04.24.537992

Figure Lengend Snippet: (A) A schematic showing the experimental design of MoNP-DiD or NP-DiD administration. (B) Representative fluorescent images of the arterial tissues isolated from ApoE −/− mice receiving MoNP-DiD or NP-DiD, with quantification of the fluorescent intensity measured in LCA, RCA, aortic arch (AA), and descending aorta (DA). Red: MoNP-DiD or NP-DiD. (C) Representative fluorescent images of RCA and LCA cross-sections from the mouse receiving MoNP-DiD. Red: MoNP-DiD, green: CD31 and elastic layer autofluorescence, and blue: nuclei. The graph indicates the intensity of DiD signal of the crosslines (yellow). Scale bar = 200 μm. (D) The fluorescent intensity of the major organs isolated from ApoE −/− mice receiving MoNP-DiD or NP-DiD. n = 6 for MoNP-DiD, and n = 4 for NP-DiD. *p < 0.05 vs. the NP-DiD group.

Article Snippet: Subsequently, the left common CA was embedded in Tissue-Tek O.C.T. compound, cryosectioned, and stained with a CD31 antibody (R&D Systems #AF3628) and DAPI.

Techniques: Isolation

Journal: Neoplasia (New York, N.Y.)

Article Title: Activated Natural Killer Cells Mediate the Suppressive Effect of Interleukin-4 on Tumor Development via STAT6 Activation in an Atopic Condition Melanoma Model 1 2

doi: 10.1016/j.neo.2017.02.014

Figure Lengend Snippet: Allergy responses inhibited IL-4–mediated tumor growth in a melanoma model. (A) Tumor growth inhibition (as assessed by tumor weight and volume) in B16F10-bearing PA-treated IL-4 mice and AOO-treated IL-4 control mice. The tumor burden was measured twice per week using a caliper, and the volume was calculated according to the following formula: volume length (mm) × width (mm) × height (mm)/2. Tumor weight and volume are presented as means ± SD from eight mice. (B) Immunohistochemistry was used to determine the expression levels of proliferating cell nuclear antigen in the melanoma tissues of PA-treated IL-4 mice and AOO-treated IL-4 mice. (C) The expression of IL-4 and apoptotic proteins was detected by Western blotting using specific antibodies: IL-4, IL-4Rα, JAK1, p-JAK1, cleaved caspase-3, -8, -9, BCL-2, and β-actin; β-actin was used as an internal control. (D) STAT6 activity in tumor tissues was detected by EMSA, and (E) the nuclear location of STAT6 was determined by Western blotting; histone protein was used as an internal control. All values represent the mean ± SD from eight animal tumor sections. * ( P < .05) indicates a significant difference from the AOO group.

Article Snippet: B16F10 mouse melanoma cells, HaCaT human keratinocytes, and NK-92MI human NK cells were obtained from the American Type Culture Collection (Cryosite, Lane Cove, NSW, Australia).

Techniques: Inhibition, Control, Immunohistochemistry, Expressing, Western Blot, Activity Assay